5425R High-liwiro Frozen Centrifuge

Amen's 5425R high-liwiro yozizira centrifuge (Centrifuge5425R), mtundu wa batani, mtundu wachisanu, rotor FA-24X2, 230V / 50-60Hz (CN), mawonekedwe opangira batani, kuyeretsa kosavuta. Kuzizira.

Amen's 5425R high-liwiro yozizira centrifuge (Centrifuge5425R), mtundu wa batani, mtundu wachisanu, rotor FA-24X2, 230V / 50-60Hz (CN), mawonekedwe opangira batani, kuyeretsa kosavuta. Kuzizira. Monga chinthu chotsatira cha Centrifuge 5424 / 5424R yotchuka, mitundu iwiri yatsopanoyi imaphatikizapo zinthu zonse zomwe mumakonda zitsanzo zam'mbuyomu, komanso ma rotor atsopano ndi mapulogalamu a mapulogalamu, zomwe zimapangitsa kuti ntchito yanu ya tsiku ndi tsiku ikhale yabwino komanso yosangalatsa. Mapangidwe apadera a mpweya wozizira wa Centrifuge5425 amaonetsetsa kuti pakhale bata kwambiri kuti pakhale malo abwino ogwirira ntchito. Kwa mphindi 12, centrifugation imatha kukwaniritsidwa, ndipo kapangidwe kake kamagwiritsidwa ntchito, ndipo phokoso lapakati ndi 45dB (a). Kuchita kwakanthawi kochepa kumeneku ndikokwanira kumaliza masitepe a centrifugation a zida zoyeretsera za DNA / RNA. Mukathamanga kwa nthawi yayitali, fani yoziziritsa imatayidwa bwino panthawi yotsalayo.

Kuwongolera kozizira kwa Centrifuge5425R kumapangitsa kuti pakhale kusiyana kwakukulu, ngakhale pa liwiro lalikulu la rotor, imatha kukhazikika bwino pa 4 ° C. Kungozizira kwa mphindi 8 (~ 21 ° C mpaka 4 ° C), centrifuge imatha kuchitidwa ntchito nthawi iliyonse. Ma rotor osiyanasiyana tsopano akugwira ntchito zosiyanasiyana, kuphatikiza ma cell achilengedwe, monga DNA / RNA kapena kuyeretsa mapuloteni, mpweya wa mabakiteriya, opangidwa kuchokera ku 5 ml yamachubu oyesa, ndi ma cell, ndi kuyesa kwa A trace, mwachitsanzo, PCR pre -mankhwala / pambuyo chithandizo ndi dongosolo QPCR amamanga mu chubu 0.2 ml, mbale 8-chitsime, kapena zigawo 96-chitsime mbale. Eppendorfkitrotor® (FA-18X2-Kit) imagwiritsidwa ntchito pamizere 18 x centrifugal ndi machubu 1.5 / 2.0ml centrifuge (osachepera 18,565 xg). Rotor yapaderayi imathetsa zovuta zomwe anthu ambiri amakumana nazo mukamagwiritsa ntchito zida zozungulira za Miniprep: Chivundikiro cha chidebe chotseguka chimadulidwa poyambira. Kawirikawiri chomwe chimachitika vutoli ndikuti ma rotor achikhalidwe sangathe kupereka chithandizo chokwanira kwa zisoti za centrifuge zomwe zimatsegulidwa panthawi ya centrifugation. Eppendorfkitrotor ili ndi m'mphepete mwake womwe umayikidwa pamenepo panthawi ya centrifugation, kotero iwo sadzadulidwa. Kuyambira pano, chivundikiro cha chidebe chanu chikhalabe patsamba lake. Rotor FA-10 × 5 imagwiritsidwa ntchito mu machubu a 10 × 5.0mleppendorf centrifuge (mpaka 21,300 xg), ndipo mabakiteriya, yisiti ya chikhalidwe kapena maselo amakololedwa mu Eppendorftubes® 5.0ml, ndi DNA / RNA ndi DNA plasmid kuyeretsa amagwiritsidwa ntchito kukhala. zosavuta kwambiri. Ma Adapter amaperekedwanso kuti akwaniritse centrifugation pogwiritsa ntchito machubu 1.5 / 2 mL centrifuge ndi HPLC ndi CRYO centrifugal chubu popanda kusintha rotor.

5425R mawonekedwe othamanga kwambiri achisanu a centrifuge:
1, chivundikiro chopanda mpweya cha EppendorfquickLock®, zitsanzo zogwirira ntchito ndizosavuta, zotetezeka;
2, mawonekedwe abwino kwambiri apulogalamu (monga mathamangitsidwe osinthika ndi malo otsetsereka, mabatani a pulogalamu 3, palibe njira zazifupi zamabatani osalekeza);
3, onjezerani pulogalamu ya rotor (mwachitsanzo, 5.0ml rotor, yoyenera mulingo wa chitoliro cha PCR);
4, OptiBowl® mapangidwe, onetsetsani kuti ntchito yosalankhula, ngakhale chivundikiro cha rotor chili chete, kanikizani pang'onopang'ono kuti mutseke chivundikiro cha centrifugal, palibe mphamvu zolimba;
5, pazipita ozungulira mphamvu: 10 × 5.0ml centrifuge chubu
6, mphamvu yaikulu yachibale centrifugal: 21,300 × g (15,060 rpm)
7,5425R Kuzizira Kwambiri Kuthamanga Kwambiri Centrifuge ntchito yapadera yogulitsa, njira yabwino kwambiri yozizirira pokonza zitsanzo za kutentha kwa 0.2-5 mL, ukadaulo wa kompresa wovomerezeka, umachepetsa kugwedezeka, kuteteza zitsanzo. Kutentha kosiyanasiyana: -10 C mpaka + 40 ° C.

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