CFX96Touch fluorescent quantitative PCR ingagwiritsidwe ntchito m'magawo osiyanasiyana ofufuza a nucleic acid quantification, kusanthula kwamtundu wa jini, kuzindikira masinthidwe amtundu, kuzindikira kwa GMO, ndi kusanthula kwazinthu zina.
Malo Ogwirira Ntchito:
1.1 Kutentha kwa ntchito: 5-31 ° C
1.2 Kugwira ntchito ndi chinyezi: chinyezi chachibale ≤80%
1.3 Mphamvu yogwira ntchito: 100-240 VAC, 50-60Hz
Kachitidwe ndi zofunikira zaukadaulo za CFX96Touch fluorescence quantitative PCR
3.1 Ntchito yayikulu (* ndiye chizindikiro chomwe chiyenera kukumana)
* 3.1.1 Njira zisanu ndi chimodzi zoyesera, 5% PCR ikhoza kuzindikirika, ndipo 5 chandamale majini akhoza kudziwika pa nthawi yomweyo, ndi wapadera FRET njira yodziwira imapezeka nthawi yomweyo.
* 3.1.2 Ndi kutentha kwamphamvu kwa PCR ntchito, mutha kuyendetsa kutentha kwa 8 nthawi imodzi, kutentha kulikonse.
3.1.3 Kutsegulidwa kwa ma reagents athunthu, kafukufuku wosiyanasiyana ndi ma reagents azachipatala amagwira ntchito;
3.1.4 Oyenera njira zosiyanasiyana za fluorescence monga Taqman, Molecular Beacon, Fret probe, Sybr Green i, etc.;
3.1.5 Open, 0.2ml single chubu, octal, 96-chitsime mbale, etc.
* 3.1.6 akhoza kuthamanga paokha, moona offline ntchito, palibe chifukwa kulumikiza kompyuta kuwunika PCR fulorosenti matalikidwe pamapindikira mu nthawi yeniyeni;
3.2 Zofunikira zazikulu zaukadaulo (* ndiye chizindikiro chomwe chiyenera kukwaniritsidwa)
* 3.2.1 mphamvu chitsanzo: 96 × 0.2ml, muyezo specifications 96-chitsime mbale (12 × 8) angagwiritsidwe ntchito;
3.2.2 Supplies Type: 0.2ml single chubu, eyiti interlocking, 96-chitsime mbale, etc.
3.2.3 Njira Yamachitidwe: 1-50μL (yovomerezeka 10-25 μL);
* 3.2.4 Gwero la kuwala: Ma LED asanu ndi limodzi okhala ndi zosefera;
* 3.2.5 Detector: Ma diode asanu ndi limodzi okhala ndi zosefera;
* 3.2.6 lita yozizira liwiro: 5 ° C / mphindi;
3.2.7 Kutentha kosiyanasiyana: 0 -100 ° C;
3.2.8 Kutentha Kwambiri: ± 0.2 ° C (90 ˚C);
3.2.9 Kutentha kofanana: ± 0.4 ° C (90 ˚C mkati mwa masekondi 10);
* 3.2.10 Dynamic Temperature Gradient Ntchito: Thamangani kutentha kwa 8 nthawi imodzi; gradient kutentha kulamulira osiyanasiyana: 30 -100 ° C; gradient kutentha kusiyana osiyanasiyana: 1 - 24 ° C; gradient kutentha makulitsidwe nthawi: chimodzimodzi;
3.2.11 chisangalalo / utsi wavelength osiyanasiyana: 450-730 nm;
3.2.12 Sensitivity: Jini imodzi yokha mu genome ya munthu imatha kuzindikirika;
3.2.13 Mtundu wamphamvu: kuchuluka kwa 10;
3.2.14 Sonyezani: 8.5 inchi mtundu kukhudza chophimba;
3.2.15 Deta Analysis Mode: Standard Curve Quantity, Melting Curve, CT kapena ΔCT Gene Expression Analysis, Multiple Internal Genodes Analysis ndi Amplification Efficiency Calculation, Multiple Data Files Gene Expression Analysis, Allelic Analysis, Type Analysis, Have Gene, kusungunuka kwa curve kusanthula ntchito. ;
3.2.16 Kutumiza Kwa data: Excel, Mawu, kapena PowerPoint. Lipoti la ogwiritsira ntchito lili ndi zoikamo, zithunzi, ndi zotsatira za deta ya tebulo, zomwe zingathe kusindikizidwa kapena kusungidwa ngati PDF;
* 3.2.17 Maphunziro a kapangidwe ka chromosomal: Njira yowunika kuchuluka kwa ma chromatin potengera gawo la kuwonongeka kwa ma genomic DNA pofanizira gawo la DNA ya genomic. Zimatsimikiziradi kulumikizana kwa kutalika pakati pa kapangidwe ka chromatin ndi mawonekedwe a jini;